Hotspot

ycf1

SSC · 126885-131900 · 5016 bp

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Pi 0.00319

MarkerSeek score 50.10

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5016
variable_sites: 16
pi: 0.00319
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 50.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	TTTACTTGGAATGGTGCCCT	AGTACCCCAAAAATGGCGAA	45.0	45.0	57.0	57.7	273 bp	1.0	70.9
ycf1_p2	ATTTACTTGGAATGGTGCCCT	AGTACCCCAAAAATGGCGAA	42.9	45.0	57.5	57.7	274 bp	1.0	67.0
ycf1_p3	TTTACTTGGAATGGTGCCCT	TGGTCTCGATTAGTACCCCA	45.0	50.0	57.0	57.2	284 bp	1.0	66.5
ycf1_p4	TTTACTTGGAATGGTGCCCT	TGGTCTCGATTAGTACCCCAA	45.0	47.6	57.0	57.9	284 bp	1.0	64.4
ycf1_p5	TTTACTTGGAATGGTGCCCT	GTACCCCAAAAATGGCGAAA	45.0	45.0	57.0	56.9	272 bp	1.0	64.4