Hotspot

trnL-UAG-ccsA

SSC · 117278-117399 · 122 bp

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Pi 0.0165

MarkerSeek score 62.80

Primer available yes

Label trnL-UAG-ccsA

Feature Metadata

label_name: trnL-UAG-ccsA
feature_type: igs
region: SSC
length_bp: 122
variable_sites: 2
pi: 0.0165
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 62.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL-UAG-ccsA_p1	GTGAAATCGGTAGACACGCT	TGGAAACCATTGAAAAGGAA	50.0	35.0	58.0	53.1	251-257 bp	1.0	42.0
trnL-UAG-ccsA_p2	TGAAATCGGTAGACACGCTG	TGGAAACCATTGAAAAGGAA	50.0	35.0	58.0	53.1	250-256 bp	1.0	41.9
trnL-UAG-ccsA_p3	CTCTTAGGAAGCAGTGCGAG	TGGAAACCATTGAAAAGGAA	55.0	35.0	58.1	53.1	230-236 bp	1.0	41.5
trnL-UAG-ccsA_p4	CTTAGGAAGCAGTGCGAGAG	TGGAAACCATTGAAAAGGAA	55.0	35.0	58.1	53.1	228-234 bp	1.0	41.5
trnL-UAG-ccsA_p5	GTGAAATCGGTAGACACGCT	TGGAAACCATTGAAAAGGAAA	50.0	33.3	58.0	54.1	251-257 bp	1.0	41.3