Hotspot

ndhF-trnL-UAG

SSC · 114885-117197 · 2313 bp

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Pi 0.00606
MarkerSeek score 55.90
Primer available yes
Label ndhF-trnL-UAG

Feature Metadata

label_name
ndhF-trnL-UAG
feature_type
igs
region
SSC
length_bp
2313
variable_sites
14
pi
0.00606
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
55.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-trnL-UAG_p1 CTGGAAGTGGAATGAAGGGT AGCGTGTCTACCGATTTCAC 50.0 50.0 57.1 58.0 2394-2520 bp 1.0 73.4
ndhF-trnL-UAG_p2 CTGGAAGTGGAATGAAGGGT CAGCGTGTCTACCGATTTCA 50.0 50.0 57.1 58.0 2395-2521 bp 1.0 73.3
ndhF-trnL-UAG_p3 CTGGAAGTGGAATGAAGGGT CTCGCACTGCTTCCTAAGAG 50.0 55.0 57.1 58.1 2415-2541 bp 1.0 72.8
ndhF-trnL-UAG_p4 CTGGAAGTGGAATGAAGGGT CTCTCGCACTGCTTCCTAAG 50.0 55.0 57.1 58.1 2417-2543 bp 1.0 72.8
ndhF-trnL-UAG_p5 CTGGAAGTGGAATGAAGGGT TCCTAAGAGCAGCGTGTCTA 50.0 50.0 57.1 57.9 2404-2530 bp 1.0 72.4