Hotspot

rpoA-rps11

LSC · 81532-81602 · 71 bp

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Pi 0.0141

MarkerSeek score 60.60

Primer available yes

Label rpoA-rps11

Feature Metadata

label_name: rpoA-rps11
feature_type: igs
region: LSC
length_bp: 71
variable_sites: 1
pi: 0.0141
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoA-rps11_p1	TTCAACACACTTCCACTGCA	CAGCATTACGGGCTATTCGT	45.0	50.0	57.9	58.1	235 bp	1.0	78.3
rpoA-rps11_p2	TTCAACACACTTCCACTGCA	CGGGCTATTCGTAGAAGTGG	45.0	55.0	57.9	57.9	227 bp	1.0	78.3
rpoA-rps11_p3	GGAGAGAGAATAAAGCGCCC	CAGCATTACGGGCTATTCGT	55.0	50.0	58.1	58.1	285 bp	1.0	78.2
rpoA-rps11_p4	GGAGAGAGAATAAAGCGCCC	CGGGCTATTCGTAGAAGTGG	55.0	55.0	58.1	57.9	277 bp	1.0	78.2
rpoA-rps11_p5	ACTGCAGTGTCCGAGTAGAT	CAGCATTACGGGCTATTCGT	50.0	50.0	58.2	58.1	221 bp	1.0	78.0