Hotspot

psaJ-rpl33

LSC · 69640-70101 · 462 bp

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Pi 0.013
MarkerSeek score 61.70
Primer available yes
Label psaJ-rpl33

Feature Metadata

label_name
psaJ-rpl33
feature_type
igs
region
LSC
length_bp
462
variable_sites
6
pi
0.013
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
61.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psaJ-rpl33_p1 ACATATCTTTCCGTGGCACC GGGCATCTTTACCCTTAGCC 50.0 55.0 58.0 58.0 598 bp 1.0 80.4
psaJ-rpl33_p2 TGGGGCTTTAGCTGGTCTAT GGGCATCTTTACCCTTAGCC 50.0 55.0 58.1 58.0 557 bp 1.0 79.9
psaJ-rpl33_p3 ACATATCTTTCCGTGGCACC TGGAAATGCCCCTTGATTCC 50.0 50.0 58.0 58.1 670 bp 1.0 79.3
psaJ-rpl33_p4 ACATATCTTTCCGTGGCACC GGAAATGCCCCTTGATTCCT 50.0 50.0 58.0 57.8 669 bp 1.0 79.1
psaJ-rpl33_p5 ACATATCTTTCCGTGGCACC TACTCGGGCATCTTTACCCT 50.0 50.0 58.0 57.8 603 bp 1.0 79.1