Hotspot

petA-psbJ

LSC · 65113-66129 · 1017 bp

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Pi 0.00591

MarkerSeek score 52.40

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 1017
variable_sites: 6
pi: 0.00591
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 52.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	CGAGAAGGTTCAATTGTCCG	TGGCATTCTTGTGATCGGTT	50.0	45.0	56.8	57.8	1124 bp	1.0	69.6
petA-psbJ_p2	CGAGAAGGTTCAATTGTCCG	ACTGGCATTCTTGTGATCGG	50.0	50.0	56.8	58.3	1126 bp	1.0	69.1
petA-psbJ_p3	CGAGAAGGTTCAATTGTCCG	CTGGCATTCTTGTGATCGGT	50.0	50.0	56.8	58.3	1125 bp	1.0	69.1
petA-psbJ_p4	CGAGAAGGTTCAATTGTCCGA	TGGCATTCTTGTGATCGGTT	47.6	45.0	58.3	57.8	1124 bp	1.0	68.4
petA-psbJ_p5	TCGAGAAGGTTCAATTGTCCG	TGGCATTCTTGTGATCGGTT	47.6	45.0	58.3	57.8	1125 bp	1.0	68.4