Hotspot

ycf4-cemA

LSC · 62239-63176 · 938 bp

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Pi 0.00535

MarkerSeek score 62.20

Primer available yes

Label ycf4-cemA

Feature Metadata

label_name: ycf4-cemA
feature_type: igs
region: LSC
length_bp: 938
variable_sites: 5
pi: 0.00535
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf4-cemA_p1	TTGGCCTATTTCTTGCGTGT	AGAGAGATCCACCAGGGTAA	45.0	50.0	58.1	56.7	1049-1051 bp	1.0	69.9
ycf4-cemA_p2	TTGGCCTATTTCTTGCGTGT	TGATAGAGAGATCCACCAGGG	45.0	52.4	58.1	57.7	1053-1055 bp	1.0	69.9
ycf4-cemA_p3	TTGGCCTATTTCTTGCGTGT	GATAGAGAGATCCACCAGGGT	45.0	52.4	58.1	57.7	1052-1054 bp	1.0	69.9
ycf4-cemA_p4	AAAAAGCTGCTGAATTGGCC	AGAGAGATCCACCAGGGTAA	45.0	50.0	57.8	56.7	1063-1065 bp	1.0	69.3
ycf4-cemA_p5	AAAAAGCTGCTGAATTGGCC	TGATAGAGAGATCCACCAGGG	45.0	52.4	57.8	57.7	1067-1069 bp	1.0	69.3