Hotspot

trnT-UGU-trnL-UAA

LSC · 47536-48401 · 866 bp

Back to Talinum

Pi 0.00462

MarkerSeek score 56.00

Primer available yes

Label trnT-UGU-trnL-UAA

Feature Metadata

label_name: trnT-UGU-trnL-UAA
feature_type: igs
region: LSC
length_bp: 866
variable_sites: 4
pi: 0.00462
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 56.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-UGU-trnL-UAA_p1	GCGATGCTCTAACCTCTGAG	AGCCAATTAAGTCCGTAGCG	55.0	50.0	57.9	58.1	939-947 bp	1.0	78.8
trnT-UGU-trnL-UAA_p2	GGAATCGAACCGATGACCAT	AGCCAATTAAGTCCGTAGCG	50.0	50.0	57.8	58.1	971-979 bp	1.0	77.9
trnT-UGU-trnL-UAA_p3	GCGATGCTCTAACCTCTGAG	CCGTAGCGTCTACCGATTTC	55.0	55.0	57.9	58.2	927-935 bp	1.0	77.3
trnT-UGU-trnL-UAA_p4	GCGATGCTCTAACCTCTGAG	GCCAATTAAGTCCGTAGCGT	55.0	50.0	57.9	58.4	938-946 bp	1.0	76.5
trnT-UGU-trnL-UAA_p5	GGAATCGAACCGATGACCAT	CCGTAGCGTCTACCGATTTC	50.0	55.0	57.8	58.2	959-967 bp	1.0	76.4