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petN-psbM

LSC · 28382-29520 · 1139 bp

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Pi 0.00439

MarkerSeek score 61.30

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 1139
variable_sites: 5
pi: 0.00439
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	ATTGTAAGTCTCGCTTGGGC	TGCTACTGCACTCTTCATTCT	50.0	42.9	58.3	57.0	1302-1306 bp	1.0	62.5
petN-psbM_p2	ATGGGGAAGAAGTGGACTCT	TGCTACTGCACTCTTCATTCT	50.0	42.9	57.6	57.0	1240-1244 bp	1.0	61.8
petN-psbM_p3	ATTGTAAGTCTCGCTTGGGC	TGCTACTGCACTCTTCATTC	50.0	45.0	58.3	55.5	1302-1306 bp	1.0	58.1
petN-psbM_p4	ATGGGGAAGAAGTGGACTCT	TGCTACTGCACTCTTCATTC	50.0	45.0	57.6	55.5	1240-1244 bp	1.0	57.4
petN-psbM_p5	CTCGCTTGGGCTGCTTTAAT	TGCTACTGCACTCTTCATTCT	50.0	42.9	58.9	57.0	1293-1297 bp	1.0	57.3