Hotspot

rpoB-trnC-GCA

LSC · 26467-27609 · 1143 bp

Back to Talinum

Pi 0.00787

MarkerSeek score 57.40

Primer available yes

Label rpoB-trnC-GCA

Feature Metadata

label_name: rpoB-trnC-GCA
feature_type: igs
region: LSC
length_bp: 1143
variable_sites: 9
pi: 0.00787
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 57.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC-GCA_p1	CCTCATTTCCATCCCGTAGC	CCGGATTTGAACTGGGGAAT	55.0	50.0	58.1	57.9	1228-1232 bp	1.0	78.5
rpoB-trnC-GCA_p2	CCTCATTTCCATCCCGTAGC	AAAGGATTTGCAGTCCTCCG	55.0	50.0	58.1	58.2	1208-1212 bp	1.0	78.3
rpoB-trnC-GCA_p3	TCCCTCATTTCCATCCCGTA	CCGGATTTGAACTGGGGAAT	50.0	50.0	57.8	57.9	1230-1234 bp	1.0	78.0
rpoB-trnC-GCA_p4	TCCCTCATTTCCATCCCGTA	AAAGGATTTGCAGTCCTCCG	50.0	50.0	57.8	58.2	1210-1214 bp	1.0	77.7
rpoB-trnC-GCA_p5	CCCTCATTTCCATCCCGTAG	CCGGATTTGAACTGGGGAAT	55.0	50.0	57.4	57.9	1229-1233 bp	1.0	75.0