Hotspot

ycf1

SSC · 122982-128015 · 5034 bp

Back to Takhtajaniantha

Pi 0.00935

MarkerSeek score 43.80

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5034
variable_sites: 47
pi: 0.00935
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 43.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	AGGAGGCCCCAAAGGATATT	TCGTCGAGGCGTAATCAAAG	50.0	50.0	58.1	58.1	646-652 bp	1.0	80.9
ycf1_p2	AGGAGGCCCCAAAGGATATT	CAGATTTTCGTCGAGGCGTA	50.0	50.0	58.1	58.1	653-659 bp	1.0	80.8
ycf1_p3	AGGAGGCCCCAAAGGATATT	AAAGAAATCCCCATTCCCCG	50.0	50.0	58.1	57.8	578-584 bp	1.0	80.5
ycf1_p4	AGGAGGCCCCAAAGGATATT	AAACCCCCTCGATGGTCATA	50.0	50.0	58.1	58.1	897-903 bp	1.0	80.4
ycf1_p5	AGGAGGCCCCAAAGGATATT	TGACTGGGATGTATCGTGGA	50.0	50.0	58.1	57.8	1190-1196 bp	1.0	79.7