Hotspot

rpl16

LSC · 81304-82693 · 1390 bp

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Pi 0.0108
MarkerSeek score 50.30
Primer available yes
Label rpl16

Feature Metadata

label_name
rpl16
feature_type
gene
region
LSC
length_bp
1390
variable_sites
15
pi
0.0108
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
50.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl16_p1 ACACATCAATTCTCGAGCCC GTCGAGGGAATTGCACGTAT 50.0 50.0 58.0 58.1 2215-2278 bp 1.0 79.9
rpl16_p2 TCGACGATTGCTAGCTCCTA GTCGAGGGAATTGCACGTAT 50.0 50.0 58.0 58.1 2245-2308 bp 1.0 79.9
rpl16_p3 GCGTCTTTGATCACAGCAAC GTCGAGGGAATTGCACGTAT 50.0 50.0 58.0 58.1 2289-2352 bp 1.0 79.8
rpl16_p4 ACACATCAATTCTCGAGCCC AATTGAAAGTCGACCCCGAG 50.0 50.0 58.0 57.9 2132-2195 bp 1.0 79.7
rpl16_p5 TCTCAATTCTCGGGCAATCG GTCGAGGGAATTGCACGTAT 50.0 50.0 58.1 58.1 2473-2536 bp 1.0 79.6