Hotspot

ndhF

SSC · 109112-111337 · 2226 bp

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Pi 0.00539

MarkerSeek score 45.50

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2226
variable_sites: 12
pi: 0.00539
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 45.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	CTAGCACTGGGTAGACCTCA	GAGGTAAGATCCGGTGAATCG	55.0	52.4	57.9	58.0	2572-2573 bp	1.0	71.4
ndhF_p2	TCTCTTCCTTCTCCGAGCTC	GAGGTAAGATCCGGTGAATCG	55.0	52.4	58.2	58.0	2702-2703 bp	1.0	70.6
ndhF_p3	CTAGCACTGGGTAGACCTCA	AGGTAAGATCCGGTGAATCG	55.0	50.0	57.9	56.8	2571-2572 bp	1.0	70.1
ndhF_p4	CTAGCACTGGGTAGACCTCA	CGAAAGAGGTAAGATCCGGTG	55.0	52.4	57.9	58.2	2577-2578 bp	1.0	70.0
ndhF_p5	CTAGCACTGGGTAGACCTCA	CGAAAGAGGTAAGATCCGGT	55.0	50.0	57.9	56.7	2577-2578 bp	1.0	69.5