Hotspot

ndhG-ndhI

SSC · 117948-118306 · 359 bp

Back to Takhtajaniantha

Pi 0.0172

MarkerSeek score 66.50

Primer available yes

Label ndhG-ndhI

Feature Metadata

label_name: ndhG-ndhI
feature_type: igs
region: SSC
length_bp: 359
variable_sites: 6
pi: 0.0172
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 66.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhG-ndhI_p1	TCCCAACGAAAAGGCAGAAT	TCAAATTGCTTTGGGTCGTT	45.0	40.0	57.7	56.4	567 bp	1.0	67.6
ndhG-ndhI_p2	TACTACTCCCAGACCCCCTA	TCAAATTGCTTTGGGTCGTT	55.0	40.0	57.7	56.4	528 bp	1.0	67.6
ndhG-ndhI_p3	ATACTACTCCCAGACCCCCT	TCAAATTGCTTTGGGTCGTT	55.0	40.0	58.4	56.4	529 bp	1.0	67.2
ndhG-ndhI_p4	AATACTACTCCCAGACCCCC	TCAAATTGCTTTGGGTCGTT	55.0	40.0	57.5	56.4	530 bp	1.0	66.2
ndhG-ndhI_p5	TCCCAACGAAAAGGCAGAAT	ATCAAATTGCTTTGGGTCGT	45.0	40.0	57.7	56.2	568 bp	1.0	65.8