Hotspot

psbE-petL

LSC · 64741-66032 · 1292 bp

Back to Takhtajaniantha

Pi 0.0126

MarkerSeek score 52.70

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 1292
variable_sites: 16
pi: 0.0126
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 52.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	TGGTCATTGAGGCCTCCTAA	AGCGCTCTTAGTTCAGTTCG	50.0	50.0	58.0	58.0	2111-2127 bp	1.0	81.1
psbE-petL_p2	CAACCCGCAATGAATAGGGA	AGCGCTCTTAGTTCAGTTCG	50.0	50.0	57.9	58.0	1947-1963 bp	1.0	81.1
psbE-petL_p3	TGGTCATTGAGGCCTCCTAA	AGAACGTGGGTCTCCAAAAC	50.0	50.0	58.0	58.0	2089-2105 bp	1.0	81.0
psbE-petL_p4	CAACCCGCAATGAATAGGGA	AGAACGTGGGTCTCCAAAAC	50.0	50.0	57.9	58.0	1925-1941 bp	1.0	81.0
psbE-petL_p5	ATTTACCCCACTTCCCTCCA	AGCGCTCTTAGTTCAGTTCG	50.0	50.0	57.9	58.0	2513-2529 bp	1.0	80.7