Hotspot

psaI-ycf4

LSC · 59510-59898 · 389 bp

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Pi 0.0154

MarkerSeek score 60.40

Primer available yes

Label psaI-ycf4

Feature Metadata

label_name: psaI-ycf4
feature_type: igs
region: LSC
length_bp: 389
variable_sites: 6
pi: 0.0154
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaI-ycf4_p1	GCAATCGCAATGGCTTCTTT	TACTTGTTTTTCGAGCCCCC	45.0	50.0	58.0	58.1	504-505 bp	1.0	80.1
psaI-ycf4_p2	GCAATCGCAATGGCTTCTTT	TCGAGCCCCCATTATAGGTT	45.0	50.0	58.0	57.9	494-495 bp	1.0	80.1
psaI-ycf4_p3	GCAATCGCAATGGCTTCTTT	TTCGAGCCCCCATTATAGGT	45.0	50.0	58.0	57.9	495-496 bp	1.0	80.1
psaI-ycf4_p4	GCAATCGCAATGGCTTCTTT	GGATAACAGCCCAGCAGAAA	45.0	50.0	58.0	57.9	525-526 bp	1.0	79.9
psaI-ycf4_p5	GCAATCGCAATGGCTTCTTT	AAAGGATAACAGCCCAGCAG	45.0	50.0	58.0	57.9	528-529 bp	1.0	79.8