Hotspot

accD-psaI

LSC · 58680-59398 · 719 bp

Back to Takhtajaniantha

Pi 0.0232

MarkerSeek score 65.70

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 719
variable_sites: 16
pi: 0.0232
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 65.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	AAAAAGCGTTCTGAGTGAGT	AAAGAAGCCATTGCGATTGC	40.0	45.0	55.5	58.0	828-857 bp	1.0	64.5
accD-psaI_p2	AAAAGCGTTCTGAGTGAGTT	AAAGAAGCCATTGCGATTGC	40.0	45.0	55.5	58.0	827-856 bp	1.0	64.5
accD-psaI_p3	TCAACTCCACACTTTCTTTCCT	AAAGAAGCCATTGCGATTGC	40.9	45.0	57.5	58.0	804-833 bp	1.0	64.4
accD-psaI_p4	AAAAAGCGTTCTGAGTGAGTT	AAAGAAGCCATTGCGATTGC	38.1	45.0	56.3	58.0	828-857 bp	1.0	62.8
accD-psaI_p5	AAAAGCGTTCTGAGTGAGTT	TTGCGATTGCCGGAAATACT	40.0	45.0	55.5	58.3	817-846 bp	1.0	62.6