Hotspot

atpB-rbcL

LSC · 54506-55270 · 765 bp

Back to Takhtajaniantha

Pi 0.00786

MarkerSeek score 63.50

Primer available yes

Label atpB-rbcL

Feature Metadata

label_name: atpB-rbcL
feature_type: igs
region: LSC
length_bp: 765
variable_sites: 6
pi: 0.00786
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpB-rbcL_p1	ATACGCCCCAGTTTCGTTTT	CACCAGCTTTGAATCCAACAC	45.0	47.6	58.1	58.0	882-889 bp	1.0	71.8
atpB-rbcL_p2	GCCTGGCGGAAAAGCTATAT	CACCAGCTTTGAATCCAACAC	50.0	47.6	58.1	58.0	928-935 bp	1.0	71.7
atpB-rbcL_p3	ATACGCCCCAGTTTCGTTTT	ACCAGCTTTGAATCCAACACT	45.0	42.9	58.1	58.1	881-888 bp	1.0	71.5
atpB-rbcL_p4	GCCTGGCGGAAAAGCTATAT	ACCAGCTTTGAATCCAACACT	50.0	42.9	58.1	58.1	927-934 bp	1.0	71.5
atpB-rbcL_p5	ATACGCCCCAGTTTCGTTTT	ACACCAGCTTTGAATCCAACA	45.0	42.9	58.1	58.3	883-890 bp	1.0	69.2