Hotspot

psaA-ycf3

LSC · 41317-42038 · 722 bp

Back to Takhtajaniantha

Pi 0.00839

MarkerSeek score 64.50

Primer available yes

Label psaA-ycf3

Feature Metadata

label_name: psaA-ycf3
feature_type: igs
region: LSC
length_bp: 722
variable_sites: 6
pi: 0.00839
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 64.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA-ycf3_p1	GAGAAATGACCCGGTCTAGC	CCAAGCCGCTGAATATTGGA	55.0	50.0	57.8	58.3	915-917 bp	1.0	76.8
psaA-ycf3_p2	TTTTACTTCTGGTTCCGGCG	CCAAGCCGCTGAATATTGGA	50.0	50.0	58.5	58.3	850-852 bp	1.0	74.8
psaA-ycf3_p3	GAGAAATGACCCGGTCTAGC	ACCAAGCCGCTGAATATTGG	55.0	50.0	57.8	58.6	916-918 bp	1.0	74.5
psaA-ycf3_p4	GAGAAATGACCCGGTCTAGC	CGACCAAGCCGCTGAATATT	55.0	50.0	57.8	58.7	918-920 bp	1.0	73.7
psaA-ycf3_p5	GAGAAATGACCCGGTCTAGC	GACCAAGCCGCTGAATATTG	55.0	50.0	57.8	57.2	917-919 bp	1.0	73.2