Hotspot

psbM-trnD-GUC

LSC · 10914-11572 · 659 bp

Back to Takhtajaniantha

Pi 0.00759

MarkerSeek score 63.10

Primer available yes

Label psbM-trnD-GUC

Feature Metadata

label_name: psbM-trnD-GUC
feature_type: igs
region: LSC
length_bp: 659
variable_sites: 5
pi: 0.00759
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-trnD-GUC_p1	GGAACTAAAATGAACAGTGCAGT	TTCAATAGGCAAGAGCACCG	39.1	50.0	57.6	58.3	776-786 bp	1.0	50.9
psbM-trnD-GUC_p2	AAAATGAACAGTGCAGTAGC	TTCAATAGGCAAGAGCACCG	40.0	50.0	54.5	58.3	770-780 bp	1.0	50.5
psbM-trnD-GUC_p3	GGAACTAAAATGAACAGTGCAGT	TCCCCGGGATTGTAGTTCAA	39.1	50.0	57.6	58.3	791-801 bp	1.0	50.3
psbM-trnD-GUC_p4	GGAACTAAAATGAACAGTGCAGT	TTCCCCGGGATTGTAGTTCA	39.1	50.0	57.6	58.3	792-802 bp	1.0	50.3
psbM-trnD-GUC_p5	AAAATGAACAGTGCAGTAGC	TCCCCGGGATTGTAGTTCAA	40.0	50.0	54.5	58.3	785-795 bp	1.0	49.8