Hotspot

rpl16

Genome · 84473-85835 · 1363 bp

Back to Taiwania

Pi 0.000734

MarkerSeek score 59.20

Primer available yes

Label rpl16

Feature Metadata

label_name: rpl16
feature_type: gene
region: Genome
length_bp: 1363
variable_sites: 1
pi: 0.000734
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16_p1	TAACCATCTTGGAGCAGTGC	AGAATTGTTACCTCGGCAGA	50.0	45.0	57.9	56.6	1466 bp	1.0	67.6
rpl16_p2	TAACCATCTTGGAGCAGTGC	AAGAATTGTTACCTCGGCAGA	50.0	42.9	57.9	57.3	1467 bp	1.0	65.6
rpl16_p3	TAACCATCTTGGAGCAGTGC	AGAATTGTTACCTCGGCAGAA	50.0	42.9	57.9	57.3	1466 bp	1.0	65.6
rpl16_p4	TAACCATCTTGGAGCAGTGC	AAGAATTGTTACCTCGGCAGAA	50.0	40.9	57.9	58.0	1467 bp	1.0	63.0
rpl16_p5	TAACCATCTTGGAGCAGTGC	AGAATTGTTACCTCGGCAGAAA	50.0	40.9	57.9	58.0	1466 bp	1.0	63.0