Hotspot

accD

Genome · 117225-119444 · 2220 bp

Back to Taiwania

Pi 0.000451

MarkerSeek score 57.40

Primer available yes

Label accD

Feature Metadata

label_name: accD
feature_type: gene
region: Genome
length_bp: 2220
variable_sites: 1
pi: 0.000451
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 57.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD_p1	AAAGAGCTCGTGGAACCATG	ACCGAATGAATAGGATGAAAA	50.0	33.3	58.2	52.9	2363-2458 bp	1.0	40.0
accD_p2	AAAGAGCTCGTGGAACCATG	ACCGAATGAATAGGATGAAAAA	50.0	31.8	58.2	53.8	2363-2458 bp	1.0	40.0
accD_p3	AAAAGAGCTCGTGGAACCAT	ACCGAATGAATAGGATGAAAA	45.0	33.3	57.4	52.9	2364-2459 bp	1.0	40.0
accD_p4	AAAGAGCTCGTGGAACCATG	TGGATTTTTACTGCTTAAAGTTGT	50.0	29.2	58.2	55.5	2304-2399 bp	1.0	40.0
accD_p5	AAAAGAGCTCGTGGAACCAT	ACCGAATGAATAGGATGAAAAA	45.0	31.8	57.4	53.8	2364-2459 bp	1.0	40.0