Hotspot

trnI-GAU-rrn16

Genome · 63812-64877 · 1066 bp

Back to Taiwania

Pi 0.00107

MarkerSeek score 62.10

Primer available yes

Label trnI-GAU-rrn16

Feature Metadata

label_name: trnI-GAU-rrn16
feature_type: igs
region: Genome
length_bp: 1066
variable_sites: 1
pi: 0.00107
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.88
markerseek_score: 62.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnI-GAU-rrn16_p1	GGCACAACGACGCAATTATC	CAAGGTAGCCGTACTGGAAG	50.0	55.0	58.2	57.7	1028-1160 bp	1.0	80.2
trnI-GAU-rrn16_p2	GGCACAACGACGCAATTATC	AGTGACTGGAGTGAAGTCGT	50.0	50.0	58.2	58.3	1050-1182 bp	1.0	80.1
trnI-GAU-rrn16_p3	GGCACAACGACGCAATTATC	AAGTCGTAACAAGGTAGCCG	50.0	50.0	58.2	57.6	1037-1169 bp	1.0	79.7
trnI-GAU-rrn16_p4	GGCACAACGACGCAATTATC	AACAAGGTAGCCGTACTGGA	50.0	50.0	58.2	58.4	1030-1162 bp	1.0	79.6
trnI-GAU-rrn16_p5	GGCACAACGACGCAATTATC	ACAAGGTAGCCGTACTGGAA	50.0	50.0	58.2	58.4	1029-1161 bp	1.0	79.6