Hotspot

rrn5-rrn4.5

Genome · 58485-58786 · 302 bp

Back to Taiwania

Pi 0.00331

MarkerSeek score 54.40

Primer available yes

Label rrn5-rrn4.5

Feature Metadata

label_name: rrn5-rrn4.5
feature_type: igs
region: Genome
length_bp: 302
variable_sites: 1
pi: 0.00331
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rrn5-rrn4.5_p1	GATTGGTGTGGTTCCCCTAC	GCCAGCGATTAGACGATAGG	55.0	55.0	57.9	58.0	423 bp	1.0	79.1
rrn5-rrn4.5_p2	AAGTTCGGGATGGATTGGTG	GCCAGCGATTAGACGATAGG	50.0	55.0	57.9	58.0	435 bp	1.0	79.0
rrn5-rrn4.5_p3	ACCAAGTTCGGGATGGATTG	GCCAGCGATTAGACGATAGG	50.0	55.0	57.9	58.0	438 bp	1.0	79.0
rrn5-rrn4.5_p4	CAAGTTCGGGATGGATTGGT	GCCAGCGATTAGACGATAGG	50.0	55.0	57.9	58.0	436 bp	1.0	79.0
rrn5-rrn4.5_p5	CACCAAGTTCGGGATGGATT	GCCAGCGATTAGACGATAGG	50.0	55.0	57.9	58.0	439 bp	1.0	79.0