Hotspot

trnC-GCA-rpoB

Genome · 24479-25253 · 775 bp

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Pi 0

MarkerSeek score 61.20

Primer available yes

Label trnC-GCA-rpoB

Feature Metadata

label_name: trnC-GCA-rpoB
feature_type: igs
region: Genome
length_bp: 775
variable_sites: 0
pi: 0
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 61.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-GCA-rpoB_p1	AACTGGGGTTCGAGGATTTG	AGCCTTGATCGATAAAACGACA	50.0	40.9	57.8	58.1	899-919 bp	1.0	63.0
trnC-GCA-rpoB_p2	AACTGGGGTTCGAGGATTTG	GCCTTGATCGATAAAACGACA	50.0	42.9	57.8	56.7	898-918 bp	1.0	61.9
trnC-GCA-rpoB_p3	TGCCTTACCACTTGGCTATG	AGCCTTGATCGATAAAACGACA	50.0	40.9	57.6	58.1	871-891 bp	1.0	61.3
trnC-GCA-rpoB_p4	GAACTGGGGTTCGAGGATTT	AGCCTTGATCGATAAAACGACA	50.0	40.9	57.5	58.1	900-920 bp	1.0	60.7
trnC-GCA-rpoB_p5	TGCCTTACCACTTGGCTATG	GCCTTGATCGATAAAACGACA	50.0	42.9	57.6	56.7	870-890 bp	1.0	60.2