Hotspot

trnK-UUU-rps16

Genome · 3705-5284 · 1580 bp

Back to Taiwania

Pi 0.000633

MarkerSeek score 58.90

Primer available yes

Label trnK-UUU-rps16

Feature Metadata

label_name: trnK-UUU-rps16
feature_type: igs
region: Genome
length_bp: 1580
variable_sites: 1
pi: 0.000633
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-rps16_p1	AGTCGCACTTAAAAGCCGAG	CGATGTGGTAGAAAGCAACG	50.0	50.0	58.6	57.2	1655 bp	1.0	69.2
trnK-UUU-rps16_p2	TAGTCGCACTTAAAAGCCGA	CGATGTGGTAGAAAGCAACG	45.0	50.0	57.3	57.2	1656 bp	1.0	67.9
trnK-UUU-rps16_p3	TCGCACTTAAAAGCCGAGTA	CGATGTGGTAGAAAGCAACG	45.0	50.0	57.3	57.2	1653 bp	1.0	67.9
trnK-UUU-rps16_p4	CGCACTTAAAAGCCGAGTAC	CGATGTGGTAGAAAGCAACG	50.0	50.0	57.2	57.2	1652 bp	1.0	67.5
trnK-UUU-rps16_p5	AGTCGCACTTAAAAGCCGAG	TGTGGTAGAAAGCAACGTGC	50.0	50.0	58.6	59.1	1652 bp	1.0	67.0