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matK-trnK-UUU

Genome · 3058-3667 · 610 bp

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Pi 0.00328

MarkerSeek score 60.60

Primer available yes

Label matK-trnK-UUU

Feature Metadata

label_name: matK-trnK-UUU
feature_type: igs
region: Genome
length_bp: 610
variable_sites: 2
pi: 0.00328
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
matK-trnK-UUU_p1	TGCGTAAAGATCTTCCCGAA	ACTGCGTATTCCACTTCGAC	45.0	50.0	57.0	58.0	772 bp	1.0	71.8
matK-trnK-UUU_p2	TGCGTAAAGATCTTCCCGAA	GCGTATTCCACTTCGACTGT	45.0	50.0	57.0	58.0	769 bp	1.0	71.8
matK-trnK-UUU_p3	TTGCGTAAAGATCTTCCCGA	ACTGCGTATTCCACTTCGAC	45.0	50.0	57.0	58.0	773 bp	1.0	71.8
matK-trnK-UUU_p4	TTGCGTAAAGATCTTCCCGA	GCGTATTCCACTTCGACTGT	45.0	50.0	57.0	58.0	770 bp	1.0	71.8
matK-trnK-UUU_p5	TGCGTAAAGATCTTCCCGAA	TGCGTATTCCACTTCGACTG	45.0	50.0	57.0	58.0	770 bp	1.0	71.8