Hotspot

psbA

LSC · 215-1276 · 1062 bp

Pi 0.00377

MarkerSeek score 35.00

Primer available yes

Label psbA

Feature Metadata

label_name: psbA
feature_type: gene
region: LSC
length_bp: 1062
variable_sites: 6
pi: 0.00377
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 35.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbA_p1	AGCAATCCCCCAAGATCTTG	GTGCTTGGGAGTCCTTGAAA	50.0	50.0	57.6	58.0	1207-1216 bp	1.0	77.0
psbA_p2	CAAGATCTTGGGGGATTGCT	GTGCTTGGGAGTCCTTGAAA	50.0	50.0	57.6	58.0	1197-1206 bp	1.0	77.0
psbA_p3	AGCAATCCCCCAAGATCTTG	TCGTGTGCAATGAAAACTGC	50.0	45.0	57.6	57.9	1525-1537 bp	1.0	76.1
psbA_p4	CAAGATCTTGGGGGATTGCT	TCGTGTGCAATGAAAACTGC	50.0	45.0	57.6	57.9	1515-1527 bp	1.0	76.1
psbA_p5	CAAGATCTTGGGGGATTGCT	AATGAAAACTGCAAGCACGG	50.0	45.0	57.6	57.9	1507-1519 bp	1.0	76.0