Hotspot

trnI-GAU-rrn16

IRa · 138722-139023 · 302 bp

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Pi 0.0265
MarkerSeek score 69.40
Primer available yes
Label trnI-GAU-rrn16

Feature Metadata

label_name
trnI-GAU-rrn16
feature_type
igs
region
IRa
length_bp
302
variable_sites
12
pi
0.0265
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
69.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnI-GAU-rrn16_p1 GGCACAACGACGCAATTATC CAAGGTAGCCGTACTGGAAG 50.0 55.0 58.2 57.7 396 bp 1.0 78.5
trnI-GAU-rrn16_p2 GGCACAACGACGCAATTATC AGTGACTGGAGTGAAGTCGT 50.0 50.0 58.2 58.3 418 bp 1.0 78.3
trnI-GAU-rrn16_p3 GGCACAACGACGCAATTATC AAGTCGTAACAAGGTAGCCG 50.0 50.0 58.2 57.6 405 bp 1.0 78.0
trnI-GAU-rrn16_p4 GGCACAACGACGCAATTATC AACAAGGTAGCCGTACTGGA 50.0 50.0 58.2 58.4 398 bp 1.0 78.0
trnI-GAU-rrn16_p5 GGCACAACGACGCAATTATC ACAAGGTAGCCGTACTGGAA 50.0 50.0 58.2 58.4 397 bp 1.0 78.0