Hotspot

ccsA-ndhD

SSC · 117956-118233 · 278 bp

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Pi 0.0336
MarkerSeek score 66.80
Primer available yes
Label ccsA-ndhD

Feature Metadata

label_name
ccsA-ndhD
feature_type
igs
region
SSC
length_bp
278
variable_sites
14
pi
0.0336
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
66.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ccsA-ndhD_p1 ATGCAGCTCTTTTATGCGGA TTCTCGATGGCTTCTCTTGC 45.0 50.0 58.0 58.0 1162-1174 bp 1.0 81.8
ccsA-ndhD_p2 TGCAGCTCTTTTATGCGGAT TTCTCGATGGCTTCTCTTGC 45.0 50.0 58.0 58.0 1161-1173 bp 1.0 81.8
ccsA-ndhD_p3 ATGCAGCTCTTTTATGCGGA CTCGATGGCTTCTCTTGCAT 45.0 50.0 58.0 58.0 1160-1172 bp 1.0 81.5
ccsA-ndhD_p4 TGCAGCTCTTTTATGCGGAT CTCGATGGCTTCTCTTGCAT 45.0 50.0 58.0 58.0 1159-1171 bp 1.0 81.5
ccsA-ndhD_p5 TTTTACCCAAGGTTTCGCCA TTCTCGATGGCTTCTCTTGC 45.0 50.0 57.9 58.0 1281-1293 bp 1.0 81.4