Hotspot

ndhF-rpl32

SSC · 115071-115883 · 813 bp

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Pi 0.0229
MarkerSeek score 64.10
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
813
variable_sites
26
pi
0.0229
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.97
markerseek_score
64.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 ACAGATTGGCCGAAAACCAT CTTTTTGACTGTCCGGTGGA 45.0 50.0 58.1 58.0 1096-1396 bp 1.0 83.4
ndhF-rpl32_p2 GGAGCGGAAAAAGGGGTATT CTTTTTGACTGTCCGGTGGA 50.0 50.0 57.9 58.0 970-1270 bp 1.0 83.1
ndhF-rpl32_p3 AGCAACTGCACCGACAAATA CTTTTTGACTGTCCGGTGGA 45.0 50.0 58.1 58.0 1638-1938 bp 1.0 82.3
ndhF-rpl32_p4 ATTTAGCAACTGCACCGACA CTTTTTGACTGTCCGGTGGA 45.0 50.0 58.1 58.0 1642-1942 bp 1.0 82.3
ndhF-rpl32_p5 TAGCAACTGCACCGACAAAT CTTTTTGACTGTCCGGTGGA 45.0 50.0 58.1 58.0 1639-1939 bp 1.0 82.3