Hotspot

rrn16-trnI-GAU

IRb · 104917-105218 · 302 bp

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Pi 0.0265
MarkerSeek score 69.40
Primer available yes
Label rrn16-trnI-GAU

Feature Metadata

label_name
rrn16-trnI-GAU
feature_type
igs
region
IRb
length_bp
302
variable_sites
12
pi
0.0265
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
69.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rrn16-trnI-GAU_p1 CAAGGTAGCCGTACTGGAAG GGCACAACGACGCAATTATC 55.0 50.0 57.7 58.2 396 bp 1.0 78.5
rrn16-trnI-GAU_p2 AGTGACTGGAGTGAAGTCGT GGCACAACGACGCAATTATC 50.0 50.0 58.3 58.2 418 bp 1.0 78.3
rrn16-trnI-GAU_p3 AAGTCGTAACAAGGTAGCCG GGCACAACGACGCAATTATC 50.0 50.0 57.6 58.2 405 bp 1.0 78.0
rrn16-trnI-GAU_p4 AACAAGGTAGCCGTACTGGA GGCACAACGACGCAATTATC 50.0 50.0 58.4 58.2 398 bp 1.0 78.0
rrn16-trnI-GAU_p5 ACAAGGTAGCCGTACTGGAA GGCACAACGACGCAATTATC 50.0 50.0 58.4 58.2 397 bp 1.0 78.0