Hotspot

rpl16-rps3

LSC · 85311-85494 · 184 bp

Back to Tainia

Pi 0.0217
MarkerSeek score 66.40
Primer available yes
Label rpl16-rps3

Feature Metadata

label_name
rpl16-rps3
feature_type
igs
region
LSC
length_bp
184
variable_sites
6
pi
0.0217
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
66.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl16-rps3_p1 TATAGTGGGGTCAGCCTGTT AGAGAAGGCAGGGTTCCTTT 50.0 50.0 57.7 58.5 384-394 bp 1.0 75.1
rpl16-rps3_p2 TTATAGTGGGGTCAGCCTGT AGAGAAGGCAGGGTTCCTTT 50.0 50.0 57.7 58.5 385-395 bp 1.0 75.1
rpl16-rps3_p3 TATAGTGGGGTCAGCCTGTT GAAGGCAGGGTTCCTTTACA 50.0 50.0 57.7 57.4 381-391 bp 1.0 74.9
rpl16-rps3_p4 TTATAGTGGGGTCAGCCTGT GAAGGCAGGGTTCCTTTACA 50.0 50.0 57.7 57.4 382-392 bp 1.0 74.9
rpl16-rps3_p5 TAACGAGTCACACACTGAGC AGAGAAGGCAGGGTTCCTTT 50.0 50.0 57.6 58.5 326-337 bp 1.0 73.7