Hotspot

rbcL-accD

LSC · 57840-58625 · 786 bp

Back to Tainia

Pi 0.0271
MarkerSeek score 67.00
Primer available yes
Label rbcL-accD

Feature Metadata

label_name
rbcL-accD
feature_type
igs
region
LSC
length_bp
786
variable_sites
32
pi
0.0271
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
67.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rbcL-accD_p1 CCCCCAGTGGATAATCTAGA TGTCCCCTTTTTGATGAAAACC 50.0 40.9 55.1 57.3 887-912 bp 1.0 44.8
rbcL-accD_p2 CCCCCAGTGGATAATCTAGA GTCCCCTTTTTGATGAAAACCT 50.0 40.9 55.1 57.0 886-911 bp 1.0 44.8
rbcL-accD_p3 CCCCCAGTGGATAATCTAGA TCTTTCTGTCCCCTTTTTGATGA 50.0 39.1 55.1 57.9 893-918 bp 1.0 44.8
rbcL-accD_p4 CCCCCAGTGGATAATCTAGAT TGTCCCCTTTTTGATGAAAACC 47.6 40.9 55.7 57.3 887-912 bp 1.0 44.8
rbcL-accD_p5 CCCCCAGTGGATAATCTAGA CCATAGATTCTTTCTGTCCCCT 50.0 45.5 55.1 56.9 901-926 bp 1.0 44.7