Hotspot

trnF-GAA-ndhJ

LSC · 48623-49214 · 592 bp

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Pi 0.0183
MarkerSeek score 74.80
Primer available yes
Label trnF-GAA-ndhJ

Feature Metadata

label_name
trnF-GAA-ndhJ
feature_type
igs
region
LSC
length_bp
592
variable_sites
14
pi
0.0183
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.98
markerseek_score
74.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnF-GAA-ndhJ_p1 CCTCGTGTCACCAGTTCAAA TGAAAGTTGGATAGGCTGGC 50.0 50.0 58.1 57.9 676-702 bp 1.0 83.4
trnF-GAA-ndhJ_p2 CCTCGTGTCACCAGTTCAAA ATAGGCTGGCCCTTACGTAA 50.0 50.0 58.1 58.2 666-692 bp 1.0 82.9
trnF-GAA-ndhJ_p3 GGGGTAGCTCAGTTGGTAGA TGAAAGTTGGATAGGCTGGC 55.0 50.0 58.1 57.9 712-738 bp 1.0 82.5
trnF-GAA-ndhJ_p4 GGGGTAGCTCAGTTGGTAGA ATAGGCTGGCCCTTACGTAA 55.0 50.0 58.1 58.2 702-728 bp 1.0 82.0
trnF-GAA-ndhJ_p5 CTCTGCTAGGATGATGCACG TGAAAGTTGGATAGGCTGGC 55.0 50.0 58.2 57.9 742-768 bp 1.0 81.7