Hotspot

psbC-trnS-UGA

LSC · 36166-36309 · 144 bp

Back to Tainia

Pi 0.0417
MarkerSeek score 72.50
Primer available yes
Label psbC-trnS-UGA

Feature Metadata

label_name
psbC-trnS-UGA
feature_type
igs
region
LSC
length_bp
144
variable_sites
9
pi
0.0417
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
72.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbC-trnS-UGA_p1 CCTGTTCTTTCCATGACCCC GAGAGATGGCTGAGTGGTTG 55.0 55.0 58.2 58.0 267-274 bp 1.0 81.2
psbC-trnS-UGA_p2 CCTGTTCTTTCCATGACCCC ATAGCCCCGGTCTTGAAAAC 55.0 50.0 58.2 57.9 247-254 bp 1.0 80.6
psbC-trnS-UGA_p3 AGCGGGGTTTGAAAAAGGAA GAGAGATGGCTGAGTGGTTG 45.0 55.0 58.2 58.0 304-311 bp 1.0 80.4
psbC-trnS-UGA_p4 TTCCATGACCCCTCTTAGCT GAGAGATGGCTGAGTGGTTG 50.0 55.0 57.7 58.0 259-266 bp 1.0 80.4
psbC-trnS-UGA_p5 TTGTGGGACATTTGTGGCAT GAGAGATGGCTGAGTGGTTG 45.0 55.0 58.3 58.0 347-354 bp 1.0 79.6