Hotspot

petN-psbM

LSC · 29752-30486 · 735 bp

Pi 0.0222

MarkerSeek score 68.90

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 735
variable_sites: 24
pi: 0.0222
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 68.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	GGGACATCATTCGCATGGAT	ACGTCAAAACAGTCAGCCAA	50.0	45.0	58.1	58.3	831-872 bp	1.0	83.4
petN-psbM_p2	GGGGAAGAAGTGGGCTTTAG	ACGTCAAAACAGTCAGCCAA	55.0	45.0	57.9	58.3	747-788 bp	1.0	83.4
petN-psbM_p3	TAGTAAGTCTCGCTTGGGCT	ACGTCAAAACAGTCAGCCAA	50.0	45.0	58.2	58.3	810-851 bp	1.0	83.0
petN-psbM_p4	TATGGGGAAGAAGTGGGCTT	ACGTCAAAACAGTCAGCCAA	50.0	45.0	58.3	58.3	750-791 bp	1.0	81.9
petN-psbM_p5	GGGACATCATTCGCATGGAT	TACGTCAAAACAGTCAGCCA	50.0	45.0	58.1	57.4	832-873 bp	1.0	80.6