Hotspot

ndhD

SSC · 116949-118451 · 1503 bp

Back to Taihangia

Pi 0.00333

MarkerSeek score 46.50

Primer available yes

Label ndhD

Feature Metadata

label_name: ndhD
feature_type: gene
region: SSC
length_bp: 1503
variable_sites: 5
pi: 0.00333
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 46.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD_p1	GGGAGCAGTATGGGCTAATG	AACCCGCGCTCAAAATAGAT	55.0	45.0	57.8	58.0	2090 bp	1.0	78.4
ndhD_p2	GGGAGCAGTATGGGCTAATG	CTTTACCGACAAAAACCCGC	55.0	50.0	57.8	58.0	2103 bp	1.0	78.4
ndhD_p3	CGGTGGAGTTCTCGCATTAT	AACCCGCGCTCAAAATAGAT	50.0	45.0	57.8	58.0	2149 bp	1.0	78.2
ndhD_p4	CGGTGGAGTTCTCGCATTAT	CTTTACCGACAAAAACCCGC	50.0	50.0	57.8	58.0	2162 bp	1.0	78.1
ndhD_p5	GGGAGCAGTATGGGCTAATG	TGATACCTTGGGACGGATGT	55.0	50.0	57.8	58.1	2311 bp	1.0	77.7