Hotspot

matK

LSC · 2020-3531 · 1512 bp

Back to Taihangia

Pi 0.00198

MarkerSeek score 59.90

Primer available yes

Label matK

Feature Metadata

label_name: matK
feature_type: gene
region: LSC
length_bp: 1512
variable_sites: 3
pi: 0.00198
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
matK_p1	TTCCATATTCGCATAACCAA	TGACCGAGGTATCCGTTTTT	35.0	45.0	52.6	56.9	1651 bp	1.0	40.1
matK_p2	TTCCATATTCGCATAACCAA	TTGACCGAGGTATCCGTTTT	35.0	45.0	52.6	56.9	1652 bp	1.0	40.1
matK_p3	TTTCCATATTCGCATAACCA	TGACCGAGGTATCCGTTTTT	35.0	45.0	52.6	56.9	1652 bp	1.0	40.1
matK_p4	TTTCCATATTCGCATAACCA	TTGACCGAGGTATCCGTTTT	35.0	45.0	52.6	56.9	1653 bp	1.0	40.1
matK_p5	TTTCCATATTCGCATAACCAA	TGACCGAGGTATCCGTTTTT	33.3	45.0	53.6	56.9	1652 bp	1.0	40.1