Hotspot

rpl20-rps12

LSC · 69992-70757 · 766 bp

Back to Taihangia

Pi 0.00392

MarkerSeek score 61.00

Primer available yes

Label rpl20-rps12

Feature Metadata

label_name: rpl20-rps12
feature_type: igs
region: LSC
length_bp: 766
variable_sites: 3
pi: 0.00392
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl20-rps12_p1	CTTGAATGAGCCCCTCGAAA	AAATGTCACGAAATCCCCCG	50.0	50.0	57.9	58.5	922 bp	1.0	75.0
rpl20-rps12_p2	TGAATGAGCCCCTCGAAAAG	AAATGTCACGAAATCCCCCG	50.0	50.0	57.9	58.5	920 bp	1.0	75.0
rpl20-rps12_p3	GAATGAGCCCCTCGAAAAGT	AAATGTCACGAAATCCCCCG	50.0	50.0	57.9	58.5	919 bp	1.0	75.0
rpl20-rps12_p4	GAGCCCCTCGAAAAGTTGAT	AAATGTCACGAAATCCCCCG	50.0	50.0	57.9	58.5	915 bp	1.0	75.0
rpl20-rps12_p5	AGCCCCTCGAAAAGTTGATG	AAATGTCACGAAATCCCCCG	50.0	50.0	58.2	58.5	914 bp	1.0	74.5