Hotspot

trnG-GCC-trnfM-CAU

LSC · 37580-37756 · 177 bp

Back to Taihangia

Pi 0.0169
MarkerSeek score 62.20
Primer available yes
Label trnG-GCC-trnfM-CAU

Feature Metadata

label_name
trnG-GCC-trnfM-CAU
feature_type
igs
region
LSC
length_bp
177
variable_sites
3
pi
0.0169
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
62.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnG-GCC-trnfM-CAU_p1 AGAAGATGCGGGTTCGATTC CTTGAGGTCACGGGTTCAAA 50.0 50.0 58.1 58.0 243 bp 1.0 80.2
trnG-GCC-trnfM-CAU_p2 GAGAAGATGCGGGTTCGATT CTTGAGGTCACGGGTTCAAA 50.0 50.0 58.1 58.0 244 bp 1.0 80.2
trnG-GCC-trnfM-CAU_p3 TTGCGGATATGGTCGAATGG CTTGAGGTCACGGGTTCAAA 50.0 50.0 58.1 58.0 284 bp 1.0 79.6
trnG-GCC-trnfM-CAU_p4 AGAAGATGCGGGTTCGATTC CTCAGCTCGTCTCTTCATCG 50.0 55.0 58.1 57.9 301 bp 1.0 79.5
trnG-GCC-trnfM-CAU_p5 GAGAAGATGCGGGTTCGATT CTCAGCTCGTCTCTTCATCG 50.0 55.0 58.1 57.9 302 bp 1.0 79.5