Hotspot

trnT-GGU-psbD

LSC · 32432-33691 · 1260 bp

Back to Taihangia

Pi 0.000815
MarkerSeek score 61.80
Primer available yes
Label trnT-GGU-psbD

Feature Metadata

label_name
trnT-GGU-psbD
feature_type
igs
region
LSC
length_bp
1260
variable_sites
1
pi
0.000815
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.97
markerseek_score
61.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnT-GGU-psbD_p1 ACTCATGGTGGCTACTGACT GGCCAGACCAACCTACAAAA 50.0 50.0 58.1 58.0 1450-1482 bp 1.0 81.3
trnT-GGU-psbD_p2 GTGGTAGAGTAACGCCATGG GGCCAGACCAACCTACAAAA 55.0 50.0 58.1 58.0 1392-1424 bp 1.0 81.3
trnT-GGU-psbD_p3 TCAGTGGTAGAGTAACGCCA GGCCAGACCAACCTACAAAA 50.0 50.0 58.1 58.0 1395-1427 bp 1.0 81.2
trnT-GGU-psbD_p4 GTGGTAGAGTAACGCCATGG TAGGCCAGACCAACCTACAA 55.0 50.0 58.1 58.0 1394-1426 bp 1.0 81.2
trnT-GGU-psbD_p5 ACTCATGGTGGCTACTGACT TAGGCCAGACCAACCTACAA 50.0 50.0 58.1 58.0 1452-1484 bp 1.0 81.1