Hotspot

trnR-UCU-atpA

LSC · 10443-11134 · 692 bp

Back to Taihangia

Pi 0.00578

MarkerSeek score 54.30

Primer available yes

Label trnR-UCU-atpA

Feature Metadata

label_name: trnR-UCU-atpA
feature_type: igs
region: LSC
length_bp: 692
variable_sites: 4
pi: 0.00578
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR-UCU-atpA_p1	AAAGGAGTGAAAAGCGTCCA	AAGGAAGCGGAAACCCTTTT	45.0	45.0	57.7	57.9	852-858 bp	1.0	77.0
trnR-UCU-atpA_p2	AAAGGAGTGAAAAGCGTCCA	TTACCAAGGAAGCGGAAACC	45.0	50.0	57.7	58.1	857-863 bp	1.0	76.8
trnR-UCU-atpA_p3	AAAGGAGTGAAAAGCGTCCA	CAGGACCAGATGGAACGTTT	45.0	50.0	57.7	57.8	819-825 bp	1.0	76.2
trnR-UCU-atpA_p4	AAGGAGTGAAAAGCGTCCAT	AAGGAAGCGGAAACCCTTTT	45.0	45.0	57.4	57.9	851-857 bp	1.0	75.2
trnR-UCU-atpA_p5	AGGAGTGAAAAGCGTCCATT	AAGGAAGCGGAAACCCTTTT	45.0	45.0	57.4	57.9	850-856 bp	1.0	75.2