Hotspot

trnK-UUU-rps16

LSC · 4312-5419 · 1108 bp

Back to Taihangia

Pi 0.00632

MarkerSeek score 62.40

Primer available yes

Label trnK-UUU-rps16

Feature Metadata

label_name: trnK-UUU-rps16
feature_type: igs
region: LSC
length_bp: 1108
variable_sites: 7
pi: 0.00632
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-rps16_p1	AGTCGCGGTCTTACAAACTC	GGGCAGGGGTTTTTAAGGAA	50.0	50.0	57.9	58.0	1263-1288 bp	1.0	79.8
trnK-UUU-rps16_p2	GTCGCGGTCTTACAAACTCT	GGGCAGGGGTTTTTAAGGAA	50.0	50.0	57.9	58.0	1262-1287 bp	1.0	79.8
trnK-UUU-rps16_p3	AGTCGCGGTCTTACAAACTC	AAGGGCAGGGGTTTTTAAGG	50.0	50.0	57.9	58.0	1265-1290 bp	1.0	79.8
trnK-UUU-rps16_p4	GTCGCGGTCTTACAAACTCT	AAGGGCAGGGGTTTTTAAGG	50.0	50.0	57.9	58.0	1264-1289 bp	1.0	79.8
trnK-UUU-rps16_p5	AAGCCGAGTACTCTACCGTT	GGGCAGGGGTTTTTAAGGAA	50.0	50.0	58.2	58.0	1179-1204 bp	1.0	79.0