Hotspot

ycf1

SSC · 122380-127428 · 5049 bp

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Pi 0.0115

MarkerSeek score 45.20

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5049
variable_sites: 58
pi: 0.0115
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 45.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	AGTTGTAGAGCGATTCTGCG	TCACAGGCAGAAGAAGAAAGA	50.0	42.9	58.1	56.9	254-260 bp	1.0	65.7
ycf1_p2	AGTTGTAGAGCGATTCTGCG	TCACAGGCAGAAGAAGAAAGAA	50.0	40.9	58.1	57.6	254-260 bp	1.0	63.4
ycf1_p3	AGTTGTAGAGCGATTCTGCG	TTCACAGGCAGAAGAAGAAAGA	50.0	40.9	58.1	57.6	255-261 bp	1.0	63.4
ycf1_p4	AGTTGTAGAGCGATTCTGCG	CACAGGCAGAAGAAGAAAGA	50.0	45.0	58.1	55.3	253-259 bp	1.0	61.2
ycf1_p5	AGTTGTAGAGCGATTCTGCG	TCACAGGCAGAAGAAGAAAG	50.0	45.0	58.1	55.3	254-260 bp	1.0	61.2