Hotspot

trnR-ACG-rrn5

IRa · 128368-128627 · 260 bp

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Pi 0.0231
MarkerSeek score 65.80
Primer available yes
Label trnR-ACG-rrn5

Feature Metadata

label_name
trnR-ACG-rrn5
feature_type
igs
region
IRa
length_bp
260
variable_sites
6
pi
0.0231
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
65.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnR-ACG-rrn5_p1 CCACGTGCTCTAATCCTCTG GGTGACGATACTGTAGGGGA 55.0 55.0 57.8 57.9 344-362 bp 1.0 79.1
trnR-ACG-rrn5_p2 CCACGTGCTCTAATCCTCTG AATCCATCCCGAACTTGGTG 55.0 50.0 57.8 57.9 379-397 bp 1.0 78.5
trnR-ACG-rrn5_p3 CCACGTGCTCTAATCCTCTG ACCAATCCATCCCGAACTTG 55.0 50.0 57.8 57.9 382-400 bp 1.0 78.5
trnR-ACG-rrn5_p4 CCACGTGCTCTAATCCTCTG CAATCCATCCCGAACTTGGT 55.0 50.0 57.8 57.9 380-398 bp 1.0 78.5
trnR-ACG-rrn5_p5 CCACGTGCTCTAATCCTCTG CACCAATCCATCCCGAACTT 55.0 50.0 57.8 57.9 383-401 bp 1.0 78.5