Hotspot

rpl32-trnL-UAG

SSC · 112143-112799 · 657 bp

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Pi 0.0268
MarkerSeek score 65.60
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
657
variable_sites
17
pi
0.0268
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.96
markerseek_score
65.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 TGTACGACAAACAAATAAGTCCT AGCGTGTCTACCGATTTCAC 34.8 50.0 56.2 58.0 707-711 bp 1.0 45.7
rpl32-trnL-UAG_p2 TGTACGACAAACAAATAAGTCCT CAGCGTGTCTACCGATTTCA 34.8 50.0 56.2 58.0 708-712 bp 1.0 45.7
rpl32-trnL-UAG_p3 TGTACGACAAACAAATAAGTCCT TCCTAAGAGCAGCGTGTCTA 34.8 50.0 56.2 57.9 717-721 bp 1.0 44.7
rpl32-trnL-UAG_p4 TGTACGACAAACAAATAAGTCCT CCTAAGAGCAGCGTGTCTAC 34.8 55.0 56.2 57.8 716-720 bp 1.0 44.5
rpl32-trnL-UAG_p5 TGTACGACAAACAAATAAGTCCT CTAAGAGCAGCGTGTCTACC 34.8 55.0 56.2 57.8 715-719 bp 1.0 44.5