Hotspot

rpl22-rps19

LSC · 83454-83534 · 81 bp

Back to Tagetes

Pi 0.0606

MarkerSeek score 70.10

Primer available yes

Label rpl22-rps19

Feature Metadata

label_name: rpl22-rps19
feature_type: igs
region: LSC
length_bp: 81
variable_sites: 4
pi: 0.0606
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.81
markerseek_score: 70.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl22-rps19_p1	GCGTATACTTCTGTTGTTGTTGT	TCCGAGGACATGCAAAAAGC	39.1	50.0	57.7	59.1	142-157 bp	1.0	50.5
rpl22-rps19_p2	GCGTATACTTCTGTTGTTGTTGT	TTCCGAGGACATGCAAAAAG	39.1	45.0	57.7	56.6	143-158 bp	1.0	48.4
rpl22-rps19_p3	AGCGTATACTTCTGTTGTTGTTG	TCCGAGGACATGCAAAAAGC	39.1	50.0	57.4	59.1	143-158 bp	1.0	48.4
rpl22-rps19_p4	GCGTATACTTCTGTTGTTGTTGT	CCGAGGACATGCAAAAAGC	39.1	52.6	57.7	57.6	141-156 bp	1.0	48.2
rpl22-rps19_p5	AGCGTATACTTCTGTTGTTGT	TCCGAGGACATGCAAAAAGC	38.1	50.0	55.3	59.1	143-158 bp	1.0	47.4