Hotspot

rpl16-rps3

LSC · 82191-82338 · 148 bp

Back to Tagetes

Pi 0.027

MarkerSeek score 68.00

Primer available yes

Label rpl16-rps3

Feature Metadata

label_name: rpl16-rps3
feature_type: igs
region: LSC
length_bp: 148
variable_sites: 4
pi: 0.027
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 68.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16-rps3_p1	AAACCAACGAGTCACACACT	GAGGGTAGGGTTCCACTACA	45.0	55.0	57.6	57.8	291-293 bp	1.0	76.0
rpl16-rps3_p2	AAACCAACGAGTCACACACT	AGAGGGTAGGGTTCCACTAC	45.0	55.0	57.6	57.5	292-294 bp	1.0	73.5
rpl16-rps3_p3	AAACCAACGAGTCACACACT	AGGGTAGGGTTCCACTACAA	45.0	50.0	57.6	57.3	290-292 bp	1.0	72.1
rpl16-rps3_p4	AACGAGTCACACACTAAGCA	GAGGGTAGGGTTCCACTACA	45.0	55.0	57.1	57.8	286-288 bp	1.0	72.1
rpl16-rps3_p5	AAACCAACGAGTCACACACT	GAGAGGGTAGGGTTCCACTA	45.0	55.0	57.6	57.2	293-295 bp	1.0	71.2