Hotspot

trnM-CAU-atpE

LSC · 51844-52056 · 213 bp

Back to Tagetes

Pi 0.0469

MarkerSeek score 79.50

Primer available yes

Label trnM-CAU-atpE

Feature Metadata

label_name: trnM-CAU-atpE
feature_type: igs
region: LSC
length_bp: 213
variable_sites: 10
pi: 0.0469
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 79.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnM-CAU-atpE_p1	TATTGCTTTCATACGGCGGG	CAAATCTAGCTCTCCGACGG	50.0	55.0	58.4	57.9	321 bp	1.0	78.2
trnM-CAU-atpE_p2	TATTGCTTTCATACGGCGGG	GGCAAATCTAGCTCTCCGAC	50.0	55.0	58.4	58.1	323 bp	1.0	78.1
trnM-CAU-atpE_p3	TATTGCTTTCATACGGCGGG	GAGGCAAATCTAGCTCTCCG	50.0	55.0	58.4	57.9	325 bp	1.0	78.0
trnM-CAU-atpE_p4	TATTGCTTTCATACGGCGGG	AGGCAAATCTAGCTCTCCGA	50.0	50.0	58.4	58.2	324 bp	1.0	77.4
trnM-CAU-atpE_p5	TATTGCTTTCATACGGCGGG	CTAGGACACGAGTAGAGGCT	50.0	55.0	58.4	57.7	300 bp	1.0	76.7